Multiple response optimization of heat shock process for separation of bovine serum albumin from plasma

Bovine serum albumin produced by heat shock from bovine plasma has been researched for the effects of sodium caprylate concentration [Cap], temperature (T), and pH on yield (Yield%) and purity (BSA%). Response surface methodology and desirability function approaches were applied to optimize its proc...

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Bibliographic Details
Published inSeparation science and technology Vol. 52; no. 12; pp. 1992 - 2001
Main Authors Gonzalez, U. A., Menendez, C., Saitua, H. A., Rigau, J.
Format Journal Article
LanguageEnglish
Published Abingdon Taylor & Francis 13.08.2017
Taylor & Francis Ltd
Subjects
Bovine serum albumin
Computer simulation
Economic feasibility
Feasibility studies
Heat shock
Monte Carlo simulation
multiple response optimization
Optimization
pH effects
Response surface methodology
Serum
Serum albumin
Simulation
Sodium
Statistical methods
Temperature effects
Yield
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Summary:Bovine serum albumin produced by heat shock from bovine plasma has been researched for the effects of sodium caprylate concentration [Cap], temperature (T), and pH on yield (Yield%) and purity (BSA%). Response surface methodology and desirability function approaches were applied to optimize its process. The best compromise solution was found with BSA% = 95.0 and Yield% = 28.5 for a [Cap] = 2 mM, T = 67.9°C, and pH = 5. A Monte Carlo simulation showed that it is possible to obtain excellent values for each individual response. However, a techno-economic feasibility study must be carried out to determine which one is the best option.
ISSN:0149-6395
1520-5754
DOI:10.1080/01496395.2017.1304421