Co-production of biosurfactant and indigo using wild-type Acinetobacter sp. isolated from soil

• Published in: Biotechnology and bioprocess engineering Vol. 29; no. 6; pp. 993 - 1002
• Main Authors: Yeo, Chan-Seo, Sudheer, Pammidimarri D. V. N., Choi, Kwon-Young
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.12.2024; Springer Nature B.V; 한국생물공학회
• Subjects: Acinetobacter; Alkanes; biosurfactants; Biotechnology; carbon; Carbon sources; Cell culture; Cell membranes; Chemical reactions; Chemistry; Chemistry and Materials Science; color; Dyes; Genetic modification; Genetically engineered organisms; Genetically modified organisms; genome; Indigo; Indoles; Industrial and Production Engineering; NMR; Nuclear magnetic resonance; Research Paper; soil; Soil investigations; solubility; Strain analysis; Surfactants; 생물공학; Biosurfactant; sp; Indole; Indigo dye
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-024-00143-0

In this research, we investigated a naturally occurring, non-genetically modified strain of Acinetobacter sp., isolated from soil, which demonstrated the capability to produce both indigo and biosurfactant. During the screening, indole was used as the sole carbon source in M9 minimal medium. The strain exhibiting the most intense blue coloration was isolated and further analyzed. The blue dye extracted from the cell culture was confirmed as indigo through LC/MS analysis, showing an m/z value of 263.5, and H-NMR analysis. In LB medium, the wild-type Acinetobacter sp. strain produced approximately 6.8 mg/L of indigo from 1 mM indole. However, in M9 minimal medium, the production yield significantly increased to 45.5 mg/L. Notably, the isolated strain showed vigorous bubbling during growth, which could facilitate the transport of indole and indigo dye, both of which have low solubility, across cell membranes. Additionally, this strain was capable of degrading medium-chain C12 alkane efficiently. The whole genome was fully sequenced and analyzed for genes concerning biosurfactant and alkane metabolisms. In conclusion, utilizing a wild-type strain for indigo production offers a promising alternative to traditional chemical processes, addressing concerns related to genetically modified organisms in future applications.