Enhanced chemiluminescence biosensor for the determination of phenolic compounds and hydrogen peroxide

The enhanced chemiluminescence (CL) reaction of the luminol–H 2O 2–horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol–gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obta...

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Published inSensors and actuators. B, Chemical Vol. 73; no. 1; pp. 71 - 75
Main Authors Ramos, M.C, Torijas, M.C, Dı́az, A.Navas
Format Journal Article
LanguageEnglish
Published Elsevier B.V 25.02.2001
Subjects
Biosensor
Chemiluminescence
Enhanced chemiluminescence
HRP
Hydrogen peroxide
Optical fibers
Optical fibre
Phenolic compounds
Phenols
Optical fibre
Phenolic compounds
Biosensor
Enhanced chemiluminescence
HRP
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Summary:The enhanced chemiluminescence (CL) reaction of the luminol–H 2O 2–horseradish peroxidase (HRP) system with immobilized HRP using microencapsulation in a sol–gel matrix has been used to develop a biosensor for p-iodophenol, p-coumaric acid, 2-naphthol and hydrogen peroxide. The detection limits obtained for p-iodophenol, p-coumaric acid and 2-naphthol were 0.83 μM, 15 and 48 nM, respectively. Direct enzyme immobilization onto the end of the optical fibre permits the construction of a remote enhanced CL biosensor. This remote biosensor has been applied to hydrogen peroxide assay (detection limit 52.2 μM and relative standard deviation 4.7% for n=4) and the results have been compared with the biosensor developed in cuvette.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(00)00681-X