Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates

A solid-phase sandwich enzyme immunoassay for serum ferritin is described. The assay procedure was simple, showed good precision, and was suitable for the routine determination of serum ferritin concentration in a clinical laboratory. The assay compared well with a conventional radioimmunoassay for...

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Bibliographic Details
Published inAnnals of clinical biochemistry Vol. 18; no. Pt 1; p. 48
Main Authors Linpisarn, S, Kricka, L J, Kennedy, J H, Whitehead, T P
Format Journal Article
LanguageEnglish
Published England 01.01.1981
Subjects
Ferritins - blood
Humans
Immunoenzyme Techniques
Radioimmunoassay
Temperature
Time Factors
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Summary:A solid-phase sandwich enzyme immunoassay for serum ferritin is described. The assay procedure was simple, showed good precision, and was suitable for the routine determination of serum ferritin concentration in a clinical laboratory. The assay compared well with a conventional radioimmunoassay for ferritin. The assay was extremely sensitive and had a detection limit of 2.2pg.
ISSN:0004-5632
DOI:10.1177/000456328101800110