An HIV-1-infected T cell clone defective in IL-2 production and Ca2+ mobilization after CD3 stimulation

• Published in: The Journal of immunology (1950) Vol. 147; no. 9; pp. 3145 - 3148
• Main Authors: Perez, VL, Rowe, T, Justement, JS, Butera, ST, June, CH, Folks, TM
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01.11.1991; American Association of Immunologists
• Subjects: AIDS/HIV; Antigens, Differentiation, T-Lymphocyte - physiology; Biological and medical sciences; Calcium - physiology; CD3 Complex; Cell Line; Dose-Response Relationship, Immunologic; Fundamental and applied biological sciences. Psychology; HIV - immunology; HIV Antigens - immunology; HIV Core Protein p24 - metabolism; HIV Infections - immunology; Humans; In Vitro Techniques; Interleukin-2 - biosynthesis; Microbiology; Receptor Aggregation; Receptors, Antigen, T-Cell - physiology; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; Signal Transduction; T-Lymphocytes - immunology; T-Lymphocytes - microbiology; Tumor Necrosis Factor-alpha - pharmacology; Virology; Human; Flow cytometry; Calcium; HIV-1 virus; Cytokine; Retroviridae; Biosynthesis; Host virus relation; Virus; Mobilization; Phenotype; Interleukin 2; T-Lymphocyte; Lentivirinae; Human immunodeficiency virus; Infected cell; Clone
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.147.9.3145

A chronically HIV-1-infected T cell clone (J1.1) derived from Jurkat cells was developed that possesses defects in CD3 signaling. This clone was phenotypically determined to be CD4- and express a reduced surface density of CD3 as compared with a pool of uninfected Jurkat clones. Although J1.1 could be induced with TNF-alpha to produce HIV-1 particles, stimulation via the CD3 (T3-Ti) complex, using mAb cross-linking, had no effect on viral production. Further investigation revealed that J1.1 secreted approximately 20-fold less IL-2 than did uninfected Jurkat cells after anti-CD3 treatment. In addition, a separate defect in Ca2+ mobilization was noted in the HIV-1-infected J1.1 line when compared with uninfected Jurkat cells after anti-CD3 cross-linking. The cell line described offers a new model in which to study the mechanisms of several defects directly imposed by HIV-1 on CD3+ cells.