Group isolation and high performance liquid chromatographic analysis of lactones in Chuanxiong

• Published in: Journal of liquid chromatography & related technologies Vol. 27; no. 3; pp. 521 - 531
• Main Authors: Ding, M.Y, Lv, W.F
• Format: Journal Article
• Language: English
• Published: Colchester Taylor & Francis Group 2004; Taylor & Francis
• Subjects: Biological and medical sciences; chemical analysis; Chuanxiong; General pharmacology; Group isolation; HPLC; Lactones; Medical sciences; medicinal plants; Oriental traditional medicine; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Solvent extraction; Asia; China; Chemical analysis; Atmospheric pressure; HPLC chromatography; Solvent extraction; Oxygen heterocycle; Medicinal plant; Membrane separation; Sample preparation; Folk medicine; Dicotyledones; Angiospermae; Bicyclic compound; Qualitative analysis; Aromatic compound; Elution; Gradient; Coupled method; Pharmacognosy; Lactone; Chemical ionization; Ultraviolet detector; Plant origin; Isolation; Spermatophyta; Umbelliferae
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-120027623

Lactones are the main active compounds of the Chinese medicinal material, Chuanxiong. A simple, sensitive, and rapid solvent extraction method was developed for the group isolation of lactones from Chuanxiong. The mixture of main lactones (senkyunolide-H, senkyunolide-I, sedanenolide, and Z-ligustilide) and the mixture of two senkyunolides (H and I) in the lactones have been isolated. A high performance liquid chromatographic method, with gradient elution, was also established for the separation and analysis of the lactones in extracted products and Chuanxiong. The identification of the lactones in the extracted products was carried out by HPLC combined with MS.