Insecticidal, biochemical and histological effects of monoterpenes against Musca domestica (Diptera: Muscidae)

• Published in: Journal of Asia-Pacific entomology Vol. 27; no. 2; pp. 102256 - 10
• Main Authors: Gharib, Ahmed M., El-Shewy, Amira M., Hamouda, Sameh S.A., Gad, Hassan A., Abdelgaleil, Samir A.M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2024; 한국응용곤충학회
• Subjects: 1,8-Cineole; Cuminaldehyde; Housefly; Mode of action; p-Cymene; Toxicity; 농학; Housefly; Cuminaldehyde; 1,8-Cineole; Toxicity; p-Cymene; Mode of action
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2024.102256

[Display omitted] •Monoterpenes are promising alternatives for Musca domestica control.•P-cymene, 1,8-cineole and cuminaldehyde were highly toxic to M. domestica larvae.•Monoterpenes induced remarkable reduction in pupation and adult emergence.•Treatment with monoterpenes altered the activity of several vital enzymes and changed some midgut histological features. The insecticidal activity of ten monoterpenes against larvae of Musca domestica (Diptera: Muscidae) was evaluated. Monoterpenes were mixed with food-media at concentrations of 2.5, 5.0, 10.0, 25.0, 50.0, 75.0 and 100.0 mg/kg. Three monoterpenes, p-cymene, 1,8-cineole and cuminaldehyde displayed a durable larvicidal activity with LC50 values of 0.14, 1.59 and 1.90 mg/kg 3 days post treatment. The three monoterpenes were more toxic than deltamethrin (LC50 = 3.36 mg/kg). Likewise, monoterpenes induced a significant reduction in pupation and adult emergence. Cuminaldehyde at 25.0 mg/kg, and p-cymene, 1,8-cineole and citronellal at 50.0 mg/kg caused a complete inhibition of pupation and adult emergence. Also, p-cymene at 25.0 mg/kg reduced the activity of larval amylase and lipase, while 1,8-cineole and cuminaldehyde increased their activity. Cuminaldehyde and α-terpinene inhibited the activity of proteases whereas p-cymene enhanced their activity. In the case of acetylcholinesterase, general-esterase and adenosinetriphosphatase, p-cymene and 1,8-cineole induced enzyme inhibition and citronellal increased enzyme activities. Finally, the tested monoterpenes caused histological changes on treated larvae, such as the basement membrane appeared and the epithelium cells deformed in some points and seen filled with scattered vacuoles. These findings indicate that the tested monoterpenes have a potential to be applied in IPM programs of M. domestica.