Heavy metal detoxication in Mytilus kidney—An in vitro study of Cd- and Zn-binding to isolated tertiary lysosomes

1. 1. Uptake of Cd and Zn by isolated lipofuschin granules (tertiary lysosomes) has been studied. 2. 2. Freshly isolated granules contain Zn and approx 85% is immobilised. 3. 3. The granules take up ionic Cd and Zn from the surrounding medium. The metals are weakly and reversibly bound by a passive,...

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Published inComparative biochemistry and physiology. C, Comparative pharmacology Vol. 76; no. 1; pp. 59 - 65
Main Author George, S.G.
Format Journal Article
LanguageEnglish
Published Oxford Elsevier B.V 1983
New York, NY Pergamon Press
Subjects
Biological and medical sciences
cadmium
Fundamental and applied biological sciences. Psychology
Invertebrates
Mollusca
Mytilus edulis
Physiology. Development
Cadmium
Mytilus edulis
Metalloprotein
Lysosome
Detoxication
Metabolism
Accumulation
Kidney
Zinc
Heavy metal
Marine environment
Pollution
Bivalvia
Invertebrata
Mollusca
Metallothionein
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Summary:1. 1. Uptake of Cd and Zn by isolated lipofuschin granules (tertiary lysosomes) has been studied. 2. 2. Freshly isolated granules contain Zn and approx 85% is immobilised. 3. 3. The granules take up ionic Cd and Zn from the surrounding medium. The metals are weakly and reversibly bound by a passive, non-concentrative adsorption process. 4. 4. Multiple, non metal-specific binding sites with a p K app ≈6 and stability constant ≈5 were found. 5. 5. Metals can be sequestered from proteins with weakly bound metal but not from Cd-thionein. 6. 6. The results are discussed in relation to intracellular control of heavy metal concentrations in the cell and the pathway of Cd metabolism.
ISSN:0306-4492
DOI:10.1016/0742-8413(83)90044-0