Defense gene expression in elicitor-treated cell suspension cultures of french bean cv. Imuna

Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone...

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Bibliographic Details
Published inPlant cell reports Vol. 8; no. 8; pp. 504 - 507
Main Authors Ellis, J.S, Jennings, A.C, Edwards, L.A, Mavandad, M, Lamb, C.J, Dixon, R.A
Format Journal Article
LanguageEnglish
Published Germany 01.12.1989
Subjects
biosynthesis
cell suspension culture
Colletotrichum lindemuthianum
cultivars
disease resistance
enzyme activity
flavonoids
gene expression
genetic control
ligases
naringenin-chalcone synthase
Phaseolus vulgaris
phenylalanine ammonia-lyase
phytoalexins
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Summary:Cell suspension cultures of bean (Phaseolus vulgaris) cv. Imuna accumulated isoflavonoid phytoalexins on exposure to elicitor from the phytopathogenic fungus Colletotrichum lindemuthianum (CL). This was preceeded by rapid increases in the activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). However, the patterns of expression of PAL and CHS genes differed from those observed in cultures of a previously studied bean cultivar. The relative levels of transcripts from individual members of the CHS multigene family differed significantly at 1.5 h compared to 22.5 h after elicitation. More strikingly, three PAL genes were expressed in cultivar Imuna in response to fungal elicitor, whereas two are expressed in elicitor-treated cell cultures of cultivar Canadian Wonder.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00269060