Hybridity and autopolyploidy increase the expressivity of apospory in diploid Paspalum rufum

• Published in: Plant systematics and evolution Vol. 302; no. 10; pp. 1471 - 1481
• Main Authors: Delgado, Luciana, Sartor, María E., Espinoza, Francisco, Soliman, Mariano, Galdeano, Florencia, Ortiz, Juan P. A.
• Format: Journal Article
• Language: English
• Published: Vienna Springer-Verlag GmbH 01.12.2016; Springer Vienna; Springer Nature B.V
• Subjects: Apomixis; Apospory; Autopolyploidy; Biomedical and Life Sciences; Colchicine; Diploids; Diploidy; Embryo sac; Embryos; Flow cytometry; Genotypes; Hybrid species; Hybrids; Life Sciences; Natural populations; Original Article; Ovaries; Ovules; Paspalum; Plant Anatomy/Development; Plant Ecology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Ploidies; Ploidy; Seeds; Tetraploidy; Apospory expressivity; Diploid; Polyploid
• ISSN: 0378-2697; 1615-6110; 2199-6881
• DOI: 10.1007/s00606-016-1345-z

Paspalum rufum Nees is a perennial grass that forms a multiploid complex. Natural populations are composed mainly of diploid (2n = 2x = 20) and tetraploid (2n = 4x = 40) cytotypes. The diploid form is sexual and highly self-sterile, while the tetraploid is pseudogamous aposporous apomict and self-fertile. Diploids can also develop aposporous sacs, and some of them complete apomixis. The objective of this work was to analyse apospory expressivity (a gametophytic apomixis component) in diploid hybrids and colchicine-induced autotetraploids of the species. One F₁ family was created by crossing two diploid individuals (R6#45 × R5#49) carrying aposporous sacs in 5.8 and 13 % of their ovules, respectively. Moreover, two synthetic autotetraploids were obtained by colchicine treatment of mature seeds from R6#45. The hybrid origin of the F₁s was confirmed by segregation analyses of a morphological trait and molecular markers, and the ploidy level of experimental plants was determined by flow cytometry. Apospory expressivity was estimated by embryo sacs observation at anthesis. Out of the 39 hybrids analysed, 38 showed aposporous embryo sacs. Expressivity of the trait ranged from 0 to 36 %, and some individuals differed significantly for both progenitors' values. Both doubled-diploid plants showed 25 and 32 % of apospory expressivity, which was significantly higher than that observed in R6#45. Results presented in this work revealed a high variability in apospory expressivity of diploid hybrids and suggested that more than one allele are controlling the trait. Moreover, the new induced doubled-diploid plants showed that apospory expressivity is highly ploidy dependent.