Novel screening assay for the detection of phenolic acid esterases

A novel plate assay method, developed for the screening of microorganisms or enzyme preparations for phenolic acid esterases, involves incorporating ethyl cinnamate into an agar medium. After inoculation and incubation, the plate is flooded with a pH-sensitive dye to reveal yellow zones around posit...

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Bibliographic Details
Published inWorld journal of microbiology & biotechnology Vol. 10; no. 1; pp. 41 - 44
Main Authors Donaghy, J.A, McKay, A.M
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 1994
Subjects
agar
Biological and medical sciences
Biotechnology
commercial enzymes
enzyme activity
Enzyme engineering
enzymes
esterases
Fundamental and applied biological sciences. Psychology
fungi
methodology
Methods. Procedures. Technologies
Miscellaneous
phenolic acids
screening
yeasts
Solid medium
Dyes
Enzyme
Phenolic acid
Cinnamic acid
Agar
Esterases
Method
Cinnamic acid(4-hydroxy 3-methoxy)
Substrate
Ester
Enzymatic activity
Microorganism culture
Hydrolases
Detection
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Summary:A novel plate assay method, developed for the screening of microorganisms or enzyme preparations for phenolic acid esterases, involves incorporating ethyl cinnamate into an agar medium. After inoculation and incubation, the plate is flooded with a pH-sensitive dye to reveal yellow zones around positive cultures against a blue background. A number of yeasts (Rhodotorula spp. and Candida spp.) and fungi (Penicillium sp. and Aspergillus sp.) gave positive results, while a number of commercial enzymes, particularly pectinases, also exhibited good phenolic acid esterase.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0959-3993
1573-0972
DOI:10.1007/BF00357561