Alpha-tocopherol-incorporating liposomes as efficient protectors from skin cell damages by single infrared-A irradiation with cold stress

• Published in: Biotechnology and bioprocess engineering Vol. 30; no. 2; pp. 275 - 286
• Main Authors: So, Chaewon, You, Gayeon, Song, Jihyeon, Yang, Jiwon, Lee, Hyeondo, Lee, Aejin, Yeo, Sangho, Kim, Su Ji, Lee, Jun Bae, Mok, Hyejung
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.04.2025; Springer Nature B.V; 한국생물공학회
• Subjects: Apoptosis; beta-galactosidase; Biotechnology; Cell adhesion & migration; Cell migration; cell movement; Chemistry; Chemistry and Materials Science; cold stress; Exposure; Fibroblasts; Galactosidase; humans; I.R. radiation; Industrial and Production Engineering; Irradiation; Keratinocytes; Liposomes; Mitochondria; photoaging; Photodegradation; photolysis; protective effect; Radiation damage; Reactive oxygen species; Research Paper; Senescence; Skin; Surface charge; Tocopherol; Vitamin E; Wound healing; α Radiation; β-Galactosidase; 생물공학; Liposome; Infrared-A ray; Cold stress; Alpha-tocopherol; Skin cells
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-024-00169-4

Considering that skin cells are exposed simultaneously to multiple stimuli in our daily life, the biological responses of skin cells to acute infrared-A (IRA) irradiation with cold stress were examined in this study. Synergistic cellular damages by single IRA exposure and cold stress (IRC) at 4 °C were investigated for human dermal fibroblasts and keratinocytes based on the cellular and mitochondrial reactive oxygen species (ROS) level, apoptotic responses, cell migration, and photoaging. After formulation of alpha-tocopherol-incorporating liposomes (Toc/Lip), their physicochemical characteristics and protective effect against IRC were investigated. Sequential exposure to IRC significantly increased the cellular and mitochondrial ROS, attenuated cell migration, elicited cellular apoptosis, and induced senescence-associated beta-galactosidase activity for fibroblasts. Toc/Lips (size of 163.6 ± 18.7 nm; surface charge of − 8.8 ± 1.8 mV) considerably attenuated the mitochondrial ROS, improved wound closure, and reduced cellular apoptosis. Finally, highly stable Toc/Lip successfully protected Toc from IRA-induced photodegradation. Single IRC exposure elicited severe biological damages to fibroblasts and keratinocytes, which was protected successfully by Toc/Lips. Accordingly, protection of skin cells using Toc/Lips might be crucial for anti-aging and healthy skin tissues in daily life.