Immunomodulatory effects of Bacillus subtilis-fermented soybean extract in mice

• Published in: Food biotechnology Vol. 36; no. 4; pp. 303 - 327
• Main Authors: Ali, Sekendar, Birhanu, Biruk Tesfaye, Lee, Eon-Bee, Quah, Yixian, Boby, Naila, Suk, Kyoungho, Lee, Sam-Pin, Lee, Seung-Jin, Park, Seung-Chun
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 02.10.2022; Taylor & Francis Ltd
• Subjects: CD18 antigen; CD4 antigen; CD8 antigen; Cytokines; Daidzein; fermentation; Fermented food; Functional foods & nutraceuticals; Immune response; Immune system; Immunoglobulin G; Immunology; Immunomodulation; Inflammation; Interleukin 2; Interleukin 4; Leukocyte migration; Leukocytes (neutrophilic); Lipopolysaccharides; Lymphocytes; Lymphocytes T; Molecular docking; Oral administration; Phagocytes; proinflammatory cytokines; Soybean; Soybeans; γ-Interferon
• ISSN: 0890-5436; 1532-4249
• DOI: 10.1080/08905436.2022.2124265

This study investigated the potential immunomodulatory effects of fermented soybean extract (FSE) in RAW264.7 cells and BALB/c mice. The expression of proinflammatory cytokines in RAW264.7 cells was measured after LPS stimulation in the presence or absence of FSE. Mice were orally administered 125, 250, and 500 mg/kg FSE for 21 days, and their immune response was analyzed. In RAW264.7 cells, FSE significantly reduced the expression of proinflammatory cytokines. In vivo experiments revealed significantly elevated splenocyte proliferation; neutrophil migration; phagocytic activities; expression of CD18 + , CD4 + , and CD8 + T cells; and expression of IL-2, IL-4, and IFN-γ. The major compounds in FSE were identified by GC-MS and by LC-MS/MS. Molecular docking analysis showed that daidzin, genistin and daidzein could potentially bind to the human high-affinity FcγRI-IgG complex to be involved in immunological responses. Taken together, FSE can be a candidate ingredient for health functional food with enhanced immunomodulating ability.