Hydrophobic PDMS promotes neural progenitor formation from SHEDs by Schwann cell-cultivated medium induction

In this study, the ability of rat Schwann cell-conditioned medium to transdifferentiate stem cells from human exfoliated deciduous teeth (SHEDs) into neural-like cells was evaluated. Furthermore, the potential effects of the hydrophobic polymer polydimethylsiloxane on neurosphere formation by the ne...

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Bibliographic Details
Published inInternational journal of polymeric materials Vol. 67; no. 1; pp. 11 - 19
Main Authors Su, Wen-Ta, Pan, Yu-Jing, Huang, Te-Yang, Huang, Yu-Ching
Format Journal Article
LanguageEnglish
Published Philadelphia Taylor & Francis 02.01.2018
Taylor & Francis Ltd
Subjects
Conditioning
Differentiation
Fluorescence
Gene expression
neural progenitor
PDMS
Phase contrast
Polydimethylsiloxane
Polymerase chain reaction
Schwann cells
Sheds
Silicone resins
Stem cells
stem cells from human exfoliated deciduous teeth
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Summary:In this study, the ability of rat Schwann cell-conditioned medium to transdifferentiate stem cells from human exfoliated deciduous teeth (SHEDs) into neural-like cells was evaluated. Furthermore, the potential effects of the hydrophobic polymer polydimethylsiloxane on neurosphere formation by the neural-like transdifferentiated SHED were investigated. The inducing effects of Rat Schwann cell-cultivated medium (RSC-CM) to SHED differentiation were investigated by inverted phase contrast microscopy. Cellular clusters formed fast at 7-day culture. Immune-fluorescent staining and neural gene expression performed by reverse transcription polymerase chain reaction showed the positive expression of nestin, tubulin III, γ-enolase, and glial fibrillary acidic protein of neural marker. Thus, RSC-CM can induce effectively neural differentiation from SHED cells.
ISSN:0091-4037
1563-535X
DOI:10.1080/00914037.2017.1297937