Cloning and expression analysis of LEAFY homologue in Pak Choi (Brassica rapa subsp. chinensis)

LEAFY is a floral meristem identity gene in Arabidopsis thaliana, which plays a key role during flowering. BrcLEAFY (BrcLFY), a FLO/LFY homologue, was cloned from Pak Choi by reverse transcription-polymerase chain reaction (RT-PCR), and its expression patterns in different organs and the apex at dif...

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Published inBiotechnology, biotechnological equipment Vol. 29; no. 6; pp. 1035 - 1042
Main Authors Hong-Xia, Song, Chao, Fu, Li-Hui, Yin, Lei-Ping, Hou, Xiao-Yong, Xu, Mei-Lan, Li
Format Journal Article
LanguageEnglish
Published Sofia Taylor & Francis 02.11.2015
Taylor & Francis Ltd
Subjects
Amino acids
Brassica
Brassica rapa subsp. chinensis
BrcLEAFY
Cloning
Cold treatment
Complementary DNA
Developmental stages
Differentiation
expression
Flowering
Gene expression
Growth stage
Homology
Low temperature
Meristems
Organs
Polymerase chain reaction
Reverse transcription
Seedlings
Seeds
Vernalization
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Summary:LEAFY is a floral meristem identity gene in Arabidopsis thaliana, which plays a key role during flowering. BrcLEAFY (BrcLFY), a FLO/LFY homologue, was cloned from Pak Choi by reverse transcription-polymerase chain reaction (RT-PCR), and its expression patterns in different organs and the apex at different developmental stages, as well as during vernalization, were analysed by quantitative real-time PCR (qPCR). The full cDNA of BrcLFY was 1353 bp in length with an open reading frame of 1260 bp encoding 419 amino acids. qPCR results showed that BrcLFY expression gradually increased during the vegetative growth stage and increased sharply at flower differentiation stage 1, with the highest expression level at flower differentiation stage 5. When comparing BrcLFY expression in different organs, we concluded that the level in young and mature leaves was significantly higher than in the apex and other tissues at flower differentiation stage 3 and visible flower bud stage and fully-bloom stage. The BrcLFY expression in germinated seeds and seedlings with five leaves was analysed during cold treatment for 0 d, 10 d and 20 d at 4 ºC and the results showed that BrcLFY gene expression rose gradually with prolonged low temperature.
ISSN:1310-2818
1314-3530
DOI:10.1080/13102818.2015.1079143