The inhibitory effects of endogenous NADPH-pyrophosphatase on the reduction of cytochrome c and the oxidation of drugs by liver microsomes

• Published in: Archives of biochemistry and biophysics Vol. 140; no. 1; pp. 113 - 121
• Main Authors: Sasame, Henry A., Gillette, James R.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.1970
• Subjects: Adenine Nucleotides; Aminopyrine; Animals; Benzopyrenes; Cytochromes - metabolism; Diphosphates - metabolism; Guinea Pigs; Kinetics; Male; Mice; Microsomes, Liver - metabolism; NADP - metabolism; Oxidoreductases - metabolism; Phosphoric Monoester Hydrolases - metabolism; Rabbits; Rats

NADPH-pyrophosphatase in rat liver converts NADPH to products which inhibit NADPH cytochrome c reductase. This reaction obscures studies on the K m (NADPH) for drug oxidation by liver microsomes from this species. Studies with mouse liver microsomes, which contain relatively little NADPH-pyrophospha...