The inhibitory effects of endogenous NADPH-pyrophosphatase on the reduction of cytochrome c and the oxidation of drugs by liver microsomes
NADPH-pyrophosphatase in rat liver converts NADPH to products which inhibit NADPH cytochrome c reductase. This reaction obscures studies on the K m (NADPH) for drug oxidation by liver microsomes from this species. Studies with mouse liver microsomes, which contain relatively little NADPH-pyrophospha...
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Published in | Archives of biochemistry and biophysics Vol. 140; no. 1; pp. 113 - 121 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.09.1970
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Subjects | |
Online Access | Get full text |
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Summary: | NADPH-pyrophosphatase in rat liver converts NADPH to products which inhibit NADPH cytochrome
c reductase. This reaction obscures studies on the
K
m
(NADPH) for drug oxidation by liver microsomes from this species. Studies with mouse liver microsomes, which contain relatively little NADPH-pyrophosphatase, revealed that the
K
m
(NADPH) for drug oxidation is less than 1 μ
m; which is at least an order of magnitude lower than that reported for aminopyrine metabolism by rat liver microsomes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-9861 1096-0384 |
DOI: | 10.1016/0003-9861(70)90015-9 |