The effects of delayed treatment with sialyl Lewis X against lipopolysaccharide-induced acute lung injury in rabbits

• Published in: European journal of pharmacology Vol. 392; no. 1; pp. 109 - 116
• Main Authors: Hayashi, Hirotsugu, Koike, Haruhiko, Imanishi, Noriaki, Tojo, Shinichiro J
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24.03.2000; Elsevier
• Subjects: Acute lung injury; Animals; Biological and medical sciences; Blood Pressure - drug effects; Capillary Permeability - drug effects; Lipopolysaccharide; Lipopolysaccharides - toxicity; Lung - drug effects; Lung - pathology; Male; Medical sciences; Neutrophils - drug effects; Neutrophils - physiology; Oligosaccharides - therapeutic use; Oxygen - blood; Pharmacology. Drug treatments; Pulmonary Gas Exchange - drug effects; Rabbits; Respiratory Distress Syndrome, Adult - drug therapy; Respiratory system; Selectin; Sialyl Lewis X; Time Factors; Lipopolysaccharide; Selectin; Acute lung injury; Sialyl Lewis X; Lung disease; Intravenous administration; Respiratory disease; Acute; Treatment efficiency; Oligosaccharide; Rabbit; Processing time; Lagomorpha; Adhesion; Vertebrata; Chemotherapy; Experimental disease; Mammalia; Bolus injection; Treatment; Animal; Inhibitor; Gas exchange
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/S0014-2999(00)00099-6

The therapeutic effects of a selectin inhibitor against lipopolysaccharide-induced acute lung injury were studied in rabbits by using sialyl Lewis X-oligosaccharide. Lipopolysaccharide-induced acute lung injury, as characterized by an impairment of pulmonary gas exchange, clinically resembles that of the acute respiratory distress syndrome. Delayed treatments with sialyl Lewis X-oligosaccharide (55 mg kg −1 i.v. bolus injection 0.5, 1 or 2 h after lipopolysaccharide administration+36 mg kg −1 h −1 i.v. infusion for 5.5, 5 or 4 h, respectively) prevented the lipopolysaccharide-induced impairments in pulmonary gas exchange, as well as the accumulation of polymorphonuclear leukocytes in the lung tissue. In contrast, this agent had no significant effects on lipopolysaccharide-induced systemic hypotension, the decrease in the number of circulating white blood cells and platelets or the decline in blood pH. This is the first demonstration that sialyl Lewis X-oligosaccharide is effective against the impairments in pulmonary gas exchange even if administered 0.5, 1 or 2 h following the lipopolysaccharide injection.