NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity

• Published in: Journal of experimental botany Vol. 70; no. 3; pp. 1033 - 1047
• Main Authors: Huang, Pin-Yao, Zhang, Jingsong, Jiang, Beier, Chan, Ching, Yu, Jhong-He, Lu, Yu-Pin, Chung, KwiMi, Zimmerli, Laurent
• Format: Journal Article
• Language: English
• Published: UK Oxford University Press 05.02.2019
• Subjects: Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Botrytis - physiology; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene Expression Regulation, Plant - immunology; Plant Diseases - immunology; Plant Immunity - genetics; Pseudomonas syringae - physiology; Repressor Proteins - genetics; Repressor Proteins - metabolism; Research Papers; Transcription Factors - genetics; Transcription Factors - metabolism; transcription factor; ethylene response factor; pattern-triggered immunity; NINJA
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jxb/ery414

Phenotypic analyses of a new ethylene response factor, ERF19, involved in the negative regulation of Arabidopsis pattern-triggered immunity. Abstract Recognition of microbe-associated molecular patterns (MAMPs) derived from invading pathogens by plant pattern recognition receptors (PRRs) initiates a subset of defense responses known as pattern-triggered immunity (PTI). Transcription factors (TFs) orchestrate the onset of PTI through complex signaling networks. Here, we characterized the function of ERF19, a member of the Arabidopsis thaliana ethylene response factor (ERF) family. ERF19 was found to act as a negative regulator of PTI against Botrytis cinerea and Pseudomonas syringae. Notably, overexpression of ERF19 increased plant susceptibility to these pathogens and repressed MAMP-induced PTI outputs. In contrast, expression of the chimeric dominant repressor ERF19-SRDX boosted PTI activation, conferred increased resistance to the fungus B. cinerea, and enhanced elf18-triggered immunity against bacteria. Consistent with a negative role for ERF19 in PTI, MAMP-mediated growth inhibition was weakened or augmented in lines overexpressing ERF19 or expressing ERF19-SRDX, respectively. Using biochemical and genetic approaches, we show that the transcriptional co-repressor Novel INteractor of JAZ (NINJA) associates with and represses the function of ERF19. Our work reveals ERF19 as a novel player in the mitigation of PTI, and highlights a potential role for NINJA in fine-tuning ERF19-mediated regulation of Arabidopsis innate immunity.