Validation of a novel immersive virtual reality set-up with responses of wild-caught freely moving coral reef fish

• Published in: Animal behaviour Vol. 206; pp. 99 - 123
• Main Authors: Vidal, Manuel, Mills, Suzanne C., Gairin, Emma, Bertucci, Frédéric, Lecchini, David
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2023; Elsevier
• Subjects: conspecific; Ecology, environment; habitat; Life Sciences; postlarval; predator; proof of concept; recruitment; reef fish; virtual reality; visual recognition; reef fish; virtual reality; habitat; predator; recruitment; conspecific; postlarval; proof of concept; visual recognition
• ISSN: 0003-3472; 1095-8282
• DOI: 10.1016/j.anbehav.2023.09.013

Virtual reality (VR) enables standardized stimuli to provoke behavioural responses in animals; however, in fish studies VR has been limited to either basic virtual simulation projected below the bowl for freely swimming individuals or a simple virtual arena rendered over a large field of view for head-restrained individuals. We developed a novel immersive VR set-up with real-time rendering of animated 3D scenarios, validated in a proof-of-concept study on the behaviour of coral reef postlarval fish. Fish use a variety of cues to select a habitat during the recruitment stage, and to recognize conspecifics and predators, but which visual cues are used remains unknown. We measured behavioural responses of groups of five convict surgeonfish, Acanthurus triostegus, to simulations of habitats, static or moving shoals of conspecifics, predators and nonaggressive heterospecifics. Postlarval fish were consistently attracted to virtual corals and conspecifics presented statically, but repulsed by their predators (bluefin jacks, Caranx melampygus). When simulated shoals repeatedly passed nearby, they were again attracted by conspecifics, showing a tendency to follow the shoal, whereas they moved repeatedly to the back of the passing predator shoal. They also discriminated between species of similar sizes: they were attracted more to conspecifics than butterflyfish, Forcipiger longirostris, and repulsed more by predators than parrotfish, Scarus psittacus. The quality of visual simulations was high enough to identify visual cues (size, body shape, colour pattern) used by postlarval fish in species recognition. Despite a tracking technology limited to fish 2D positions in the aquarium, preventing the real-time updating of the rendered viewpoint, we could show that VR and modern tracking technologies offer new possibilities to investigate fish behaviour through the quantitative analysis of their physical reactions to highly controlled scenarios. •Immersive virtual reality set-up with automated tracking for fish behaviour.•Highly realistic and well-controlled animated 3D scenarios.•Real-time collection of numerous parameters about fish kinematics.•Proof of concept study on postlarval surgeonfish, Acanthurus triostegus.•Modelling of reactions to virtual habitat, adult conspecifics and predators.