Optimization of co-culture condition with respect to aeration and glucose to xylose ratio for bioethanol production

• Published in: Indian chemical engineer (Calcutta, India : 1997) Vol. 65; no. 2; pp. 233 - 248
• Main Authors: Kumar, Shashi, Agarwal, G. P., Sreekrishnan, T. R.
• Format: Journal Article
• Language: English
• Published: New Delhi Taylor & Francis 04.03.2023; Taylor & Francis Ltd
• Subjects: Aeration; aeration rate; Bioethanol; Biofuels; co-culture; Ethanol; Glucose; glucose to xylose ratio; Glucose-Xylose; microaerobic fermentation; Optimization; Productivity; Yeast
• ISSN: 0019-4506; 0975-007X
• DOI: 10.1080/00194506.2023.2190332

The present study was designed to find a suitable microaerobic condition and ratio of glucose and xylose for maximum ethanol production using co-culture of Saccharomyces cerevisiae and Pichia stipitis. The maximum ethanol concentration and yield were achieved at 0.05 vvm aeration rate and 2:1 glucose/xylose ratio. The co-culture resulted in maximum ethanol concentration, ethanol yield, and volumetric productivity of 12.33 ± 0.10 g/L, 0.43 g/g, and 0.26 g/L/h, respectively. While, the monoculture of P. stipitis resulted in 8.96 ± 0.13 g/L, 0.36 g/g, and 0.19 g/L/h respectively. The fermentation carried out in microaerobic mode delivered 10.68% and 10.56% more ethanol concentration and ethanol yield respectively from glucose compared to the combination of anaerobic and microaerobic mode. Also, the glucose uptake rate increased to 0.83 g/L/h, which corresponds to an improvement of 50.16%, suggesting that the lower microaerophilic condition not only supports P. stipitis metabolism but also does S. cerevisiae to convert glucose faster in a co-culture system. Hence, co-culture cultivation in microaerobic mode would be a better condition to achieve maximum ethanol and productivity.