A diet high in glucose and deficient in dietary fibre causes fat accumulation in the liver without weight gain

• Published in: Biochemistry and biophysics reports Vol. 40; p. 101848
• Main Authors: Karino, Sonoko, Usuda, Haruki, Kanda, Shoma, Okamoto, Takayuki, Niibayashi, Tomomi, Yano, Takahisa, Naora, Kohji, Wada, Koichiro
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2024; Elsevier
• Subjects: de novo lipogenesis; Dietary fibre; Hepatic triglyceride; High-glucose; Dietary fibre; de novo lipogenesis; High-glucose; Hepatic triglyceride
• ISSN: 2405-5808; 2405-5808
• DOI: 10.1016/j.bbrep.2024.101848

This study investigated whether a standard calorie diet that is high in glucose and deficient in dietary fibre (described as HGD [high glucose diet]) induces hepatic fat accumulation in mice. We evaluated hepatic steatosis at 7 days and 14 days after the commencement of the HGD. Hepatic triglycerides and areas of oil droplets increased in the HGD group both at day 7 and day 14, whereas weight gain, weight of epididymal fat, and plasma levels of triglycerides were unaffected by HGD consumption. A microarray analysis of the livers revealed that the expression of lipogenesis-related genes was the most affected by HGD consumption. Furthermore, HGD consumption induced the expression of hepatic proteins of fatty acid synthetase, acetyl-CoA carboxylase alpha, and stearoyl-CoA desaturase 1, which are known to be involved in the synthesis of triglyceride. These results indicate that HGD consumption causes fat accumulation in the liver, with an increase in enzymes that are involved in de novo lipogenesis without an accompanying weight or obesity phenotype. Our new findings suggest that HGD consumption could serve as a breeding ground for liver steatosis. •A diet high in glucose and deficient in fibre (described as HGD [high glucose diet]) induces hepatic accumulation of triglyceride in a short term.•The fat accumulation in the liver does not accompanies with neither body weight gain nor increase in epididymal fat weight.•Lipogenesis-responsible enzymes including fatty acid synthase (FASN), stearoyl-CoA desaturase 1 (SCD1) and acetyl-CoA carboxylase alpha (ACCα) are suggested to be responsible factors for the fat accumulation in the liver.