Reduced Expression of Programmed Cell Death 5 Protein in Tissue of Human Prostate Cancer

• Published in: Chinese medical sciences journal Vol. 24; no. 4; pp. 241 - 245
• Main Authors: Du, Yue-jun, Xiong, Lin, Lou, Yan, Tan, Wan-long, Zheng, Shao-bin
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2009; Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China%First Department of Surgery, 187 Hospital of PLA, Haikou 571159, China%Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
• Subjects: Aged; Apoptosis; Apoptosis Regulatory Proteins - analysis; Apoptosis Regulatory Proteins - physiology; Humans; Immunohistochemistry; Male; Middle Aged; Neoplasm Proteins - analysis; Neoplasm Proteins - physiology; PDCD5; programmed cell death 5; Prostate - chemistry; prostate cancer; Prostatic Neoplasms - chemistry; Prostatic Neoplasms - etiology; Prostatic Neoplasms - pathology; Proto-Oncogene Proteins c-bcl-2 - analysis; 前列腺癌; 癌组织; 程序性细胞死亡; 细胞程序性死亡; 良性前列腺增生; 蛋白质; 计算机成像; apoptosis; prostate cancer; programmed cell death 5
• ISSN: 1001-9294
• DOI: 10.1016/S1001-9294(10)60009-0

Objective To investigate the expression of programmed cell death 5 （PDCD5） in tissues of normal human prostate （NP）, benign prostatic hyperplasia （BPH）, and prostate cancer （PCa） in order to assess the clinical role of PDCD5 in PCa. Methods PDCD5 expression was determined by EnVision immunohistochemical staining in forma-lin-fixed and paraffin-embedded specimens obtained from 12 subjects with NP, 22 with BPH, and 22 with PCa. In addition, PCa cases were classified as low/middle-risk （Gleason sumS7） and high-risk （Gleason sum〉7） on the basis of Gleason grade. Positive expression rates and intensity of PDCD5 protein were observed under light microscope and analyzed with computer imaging technique. Expression of PDCD5 was compared among different prostatic tissues. Results The expression of PDCD5 was significantly lower in tissue of PCa than in tissues of NP and BPH （P〈0.01）. However, there was no significant difference in PDCD5 expression between tissues of NP and BPH. In addition, the expression of PDCD5 was further downregulated with the increase of Gleason sum in PCa. Conclusions By downregulating apoptosis, low PDCD5 expression may play an important role in the occurrence and development of PCa. PDCD5 is supposed to have a potential clinical value to be a new predictor of progression and target of gene therapy in PCa.