Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

• Published in: Chinese medical sciences journal Vol. 25; no. 2; pp. 95 - 99
• Main Authors: Chen, Feng, Xu, Zhen, Lu, Jing, Lü, Xiang, Mu, Wen-li, Wang, Ya-jun, Liu, De-pei, Liang, Chih-chuan
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.06.2010; National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005,China
• Subjects: Animals; Arecaceae - enzymology; Cell Line; Gaussia luciferase; gene delivery systems; Gene Transfer Techniques; Genes, Reporter; Humans; Luciferases - genetics; Mice; reporter; 传递系统; 脾淋巴细胞; 荧光素酶; 血液检测; 转基因; gene delivery systems; reporter; Gaussia luciferase

Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase（Gluc） expression cassette.After implantation of these cells into recipient mice...