Gaussia Luciferase Reporter Assay for Assessment of Gene Delivery Systems in Vivo

• Published in: Chinese medical sciences journal Vol. 25; no. 2; pp. 95 - 99
• Main Authors: Chen, Feng, Xu, Zhen, Lu, Jing, Lü, Xiang, Mu, Wen-li, Wang, Ya-jun, Liu, De-pei, Liang, Chih-chuan
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.06.2010; National Laboratory of Medical Molecular Biology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100005,China
• Subjects: Animals; Arecaceae - enzymology; Cell Line; Gaussia luciferase; gene delivery systems; Gene Transfer Techniques; Genes, Reporter; Humans; Luciferases - genetics; Mice; reporter; 传递系统; 脾淋巴细胞; 荧光素酶; 血液检测; 转基因; gene delivery systems; reporter; Gaussia luciferase
• ISSN: 1001-9294
• DOI: 10.1016/S1001-9294(10)60029-6

Objective To develop an alternative method for assessment of gene delivery systems in vivo.Methods Mouse primary spleen lymphocytes were genetically modified in vitro by a retroviral vector harboring a Gaussia luciferase（Gluc） expression cassette.After implantation of these cells into recipient mice,the expression of Gluc was detected in whole blood or plasma collected.Results As little as 10 μL whole blood drawn from the recipient mice could guarantee prompt reading of Gluc activity with a luminometer.And the reading was found in good correlation with the number of genetically modified spleen lymphocytes implanted to the mice.Conclusions Gluc may be useful as an in vivo reporter for gene therapy researches,and Gluc blood assay could provide an alternative method for assessment of gene delivery systems in vivo.