Antigen-specific T-cell responses in patients with non–IgE-mediated gastrointestinal food allergy are predominantly skewed to TH2

• Published in: Journal of allergy and clinical immunology Vol. 131; no. 2; pp. 590 - 592.e6
• Main Authors: Morita, Hideaki, Nomura, Ichiro, Orihara, Kanami, Yoshida, Koichi, Akasawa, Akira, Tachimoto, Hiroshi, Ohtsuka, Yoshikazu, Namai, Yoshiyuki, Futamura, Masaki, Shoda, Tetsuo, Matsuda, Akio, Kamemura, Norio, Kido, Hiroshi, Takahashi, Takao, Ohya, Yukihiro, Saito, Hirohisa, Matsumoto, Kenji
• Format: Journal Article
• Language: English
• Published: St. Louis Mosby, Inc 01.02.2013; Elsevier Limited
• Subjects: Allergies; Cytokines; Food allergies; Milk; Studies; Japan
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2012.09.005

The limulus amebocyte lysate assay detected high concentrations of LPS in commercially available milk protein preparations, as previously reported (see Table E1 in this article's Online Repository at www.jacionline.org).3 In addition, significant lymphoproliferative responses were found in the presence of as little as 10 pg/mL LPS (see Fig E1, A, in this article's Online Repository at www.jacionline.org), and PBMCs from younger children showed more pronounced lymphoproliferation in response to LPS (see Fig E1, B). [...]we attempted to remove contaminating LPS from milk protein preparations by passing them through a prepacked endotoxin affinity column.  Proliferation (SI)[low *] 20 1.290 (0.830-1.738) 9 3.077 (2.484-3.492) 65 2.894 (2.004-7.147) <.01 <.001 Cytokine (pg/mL)         TNF-α 12 74.69 (58.44-144.8) 10 77.78 (58.04-141.4) 65 241.0 (89.21-729.6) NS <.05 IL-6 12 79.24 (36.36-193.8) 10 337.9 (57.43-1021) 65 1151 (157.0-4802) NS <.01 IL-1β 11 26.02 (6.880-46.47) 10 27.49 (6.548-65.04) 64 48.75 (11.7-136.1) NS NS IL-2 12 4.15 (0.0-10.04) 10 12.31 (7.23-17.58) 58 16.32 (7.760-39.49) NS <.01 IL-3 12 0.0 (0.0-0.38) 10 0.40 (0.0-3.61) 62 4.22 (0.0-29.49) NS <.05 IL-4§ 12 5.365 (2.895-6.358) 10 3.795 (2.033-7.788) 65 5.670 (2.775-12.06) NS NS IL-5 12 2.080 (0.0-19.56) 10 46.59 (4.663-173.5) 65 63.66 (7.360-310.4) NS <.01 IL-10 12 9.285 (3.075-15.71) 10 56.17 (18.74-76.91) 65 57.92 (12.61-198.8) NS <.05 IL-13 12 21.61 (0.270-65.04) 10 82.56 (16.28-555.3) 65 291.7 (22.10-1417) NS <.01 IFN-γ 11 3.910 (0.0-67.06) 10 31.91 (3.635-102.0) 65 71.86 (5.49-303.4) NS NS IL-17 12 0.0 (0.0-2.350) 10 7.635 (1.710-39.63) 65 7.150 (0.0-17.83) NS NS Table II Antigen-specific lymphoproliferation and cytokine production profiles in patients with gastrointestinal food allergies, patients with IgE-mediated allergy, and control subjects PBMCs from each patient were stimulated separately with each of 5 different milk protein preparations, and the data show the highest concentration of each cytokine detected in response to the 5 different stimuli.