Adamantane-Monoterpenoid Conjugates Linked via Heterocyclic Linkers Enhance the Cytotoxic Effect of Topotecan

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 11; p. 3374
• Main Authors: Munkuev, Aldar A, Dyrkheeva, Nadezhda S, Kornienko, Tatyana E, Ilina, Ekaterina S, Ivankin, Dmitry I, Suslov, Evgeniy V, Korchagina, Dina V, Gatilov, Yuriy V, Zakharenko, Alexandra L, Malakhova, Anastasia A, Reynisson, Jóhannes, Volcho, Konstantin P, Salakhutdinov, Nariman F, Lavrik, Olga I
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.05.2022; MDPI
• Subjects: 1,2,4-triazole; 1,3,4-thiadiazole; adamantane; Antitumor activity; Biosensors; Camphor; Cancer therapies; Chemotherapy; Chloride; Colorectal cancer; Conjugates; Cytotoxicity; Deoxyribonucleic acid; DNA; DNA damage; Enzymes; monoterpene; Phosphodiesterase; Sulfur; TDP1 inhibitors; Thiadiazoles; Topotecan; Toxicity; tyrosyl-DNA phosphodiesterase 1; TDP1 inhibitors; adamantane; monoterpene; synergy; 1,2,4-triazole; 1,3,4-thiadiazole; tyrosyl-DNA phosphodiesterase 1
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27113374

Inhibiting tyrosyl-DNA phosphodiesterase 1 (TDP1) is a promising strategy for increasing the effectiveness of existing antitumor therapy since it can remove the DNA lesions caused by anticancer drugs, which form covalent complexes with topoisomerase 1 (TOP1). Here, new adamantane-monoterpene conjugates with a 1,2,4-triazole or 1,3,4-thiadiazole linker core were synthesized, where (+)-and (-)-campholenic and (+)-camphor derivatives were used as monoterpene fragments. The campholenic derivatives - and - showed activity against TDP1 at a low micromolar range with IC ~5-6 μM, whereas camphor-containing compounds and were ineffective. Surprisingly, all the compounds synthesized demonstrated a clear synergy with topotecan, a TOP1 poison, regardless of their ability to inhibit TDP1. These findings imply that different pathways of enhancing topotecan toxicity other than the inhibition of TDP1 can be realized.