Analysis of cellular proteins in 9, 10-dimethyl-1, 2-benzanthracene induced hamster tongue carcinoma and dysplastic lesions by two-dimensional gel electrophoresis

• Published in: Japanese Journal of Oral Biology Vol. 32; no. 1; pp. 20 - 27
• Main Authors: Ohsaka, Fumihiro, Asanami, Souichirou, Kizaki, Harutoshi, Sakurada, Tomomi, Tanaka, Youichi
• Format: Journal Article
• Language: English; Japanese
• Published: Japan Japanese Association for Oral Biology 1990
• Subjects: 9, 10-dimethyl-1, 2-benzanthracene; Animals; Carcinoma, Squamous Cell - chemically induced; Carcinoma, Squamous Cell - chemistry; Cricetinae; dysplasia; Electrophoresis, Gel, Two-Dimensional; Hyperplasia - pathology; Isoelectric Focusing; Neoplasm Proteins - analysis; Tongue - pathology; tongue carcinoma; Tongue Neoplasms - chemically induced; Tongue Neoplasms - chemistry; two-dimensional electrophoresis
• ISSN: 0385-0137
• DOI: 10.2330/joralbiosci1965.32.20

The cellular proteins of hamster tongue squamous cell carcinoma and epitherial dysplastic lesions induced by 9, 10-dimethyl-1, 2-benzanthracend (DMBA) were analyzed by two-dimensional isoelectric focusing-sodium dodecyl sulfate gel electrophoresis. Many of the polypeptides were common to these lesions. However, there were thirteen main polypeptides which differed between normal tongue mucosa and tongue carcinoma. In the carcinoma, eight polypeptides (5.2/53K, 5.8/47K, 6.9/46K, 6.9/50K, 7.1/55K, 7.2/46K, 7.3/75K, 7.4/48K, pI/MW) were decreased and three polypeptides (4.9/32K, 6.3/50K, 7.2/60K) were increased in an amount. The remaining two polypeptides (4.8/ 37K, 4.9/36K) that were not normally present were appeared in carcinoma, but these two new polypeptides were not specific to carcinoma because they were also present in dysplastic lesions. There were marked differences in the polypeptide between various degree of dysplastic lesions and carcinoma on the one hand and normal mucosa on the others. These results suggests that twodimensional electrophoresis may have usefulness in further research on transformations from normal mucosa to malignant cancer cells.