A homogeneous label-free electrochemical aptasensor based on an omega-like DNA nanostructure for progesterone detection
A novel homogeneous label-free electrochemical aptamer sensor for the detection of progesterone was prepared by combining a well-designed omega (Ω)-like DNA (Ω-DNA) nanostructure, with an isothermal cycling amplification strategy based on the highly efficient exonuclease III (Exo III). The omega-lik...
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Published in | Analytical methods Vol. 15; no. 45; pp. 6159 - 6164 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Royal Society of Chemistry
23.11.2023
|
Subjects | |
Online Access | Get full text |
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Summary: | A novel homogeneous label-free electrochemical aptamer sensor for the detection of progesterone was prepared by combining a well-designed omega (Ω)-like DNA (Ω-DNA) nanostructure, with an isothermal cycling amplification strategy based on the highly efficient exonuclease III (Exo III). The omega-like (Ω) DNA is composed of two oligonucleotide strands: DNA
1
and DNA
2
. The Pro aptamer triggers a chain displacement reaction of Ω-DNA nanostructures, forms a new double-stranded DNA structure (aptamer precursor-DNA
2
), and releases DNA
1
. Then, Exo III selectively cleaves the DNA duplex and releases the Pro aptamer to participate in a new displacement reaction. Meanwhile, the released DNA
1
strands gain access to the strongly bound hemin, forming a hemin/G-quadruplex (DNAzyme). In the presence of hydrogen peroxide (H
2
O
2
), differential pulse voltammetry (DPV) was used to detect the current signal from the oxidation of
o
-phenylenediamine (OPD) to aminoazobenzene (DAP) catalyzed by DNAzyme. However, the amount of released DNA
1
from the Ω-DNA nanostructures is reduced in the presence of the target Pro, and the DPV signal declines because of the small amount of DNAzyme formed. The developed electrochemical aptasensor has a wide dynamic linear relationship in the range of 1 pg mL
−1
to 10 ng mL
−1
under optimal conditions. Its detection limit is down to 0.3 pg mL
−1
, providing a potential platform for a sensitive Pro assay among electrochemical assays.
A novel homogeneous label-free electrochemical aptamer sensor for the detection of progesterone was prepared by combining the omega-like DNA nanostructure, with an isothermal cycling amplification strategy based on the exonuclease III. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1759-9660 1759-9679 |
DOI: | 10.1039/d3ay01255g |