Improvement in phloroglucinol compound extracted from different parts of Eugenia umbelliflora monitored by LC-UV and antimicrobial activity

• Published in: Natural product research Vol. 36; no. 14; pp. 1 - 3716
• Main Authors: Farias, Ingrid Vicente, Amorim, Clarissa Medeiros de, Graff, Eliane, Breia, Isabel, Dos Santos, Matheus Corrêa, Norberto, Silvana, Faqueti, Larissa G, Bresolin, Tania Mari Bellé, Bella Cruz, Alexandre, Meyre-Silva, Christiane
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Ltd 01.07.2022
• Subjects: Antiinfectives and antibacterials; Antimicrobial activity; Ethanol; Fruits; Phloroglucinol; Raw materials; Room temperature; Solvents; herbal drug; LC-UV; biomonitoring; Eugenial; extraction optimization
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2020.1868458

The phloroglucinol eugenial C, eugenial D and eugenial E are the main active compounds in fruits. This study aims to evaluate the extraction conditions of , using ethanol as solvent, focusing on the phloroglucinol and antimicrobial activity. In order to optimize the extraction conditions, ethanol 50, 70 and 90 GL was used as a solvent in the proportions of 1:20 (w/v) of drug:solvent ratio (D:S), stirring (330 rpm) at room temperature during 4 h, monitored by LC-UV and antimicrobial assay. The LC-UV method developed was linear over a concentration range of 3.4-68.0, 5.3-106.0 and 5.0-100.8 μg.mL of eugenial C, eugenial D and eugenial E, having LOQ of 1.68, 1.33 and 0.8 μg.mL , respectively. The fruits showed the best herbal raw material and showed the highest phloroglucinol concentration and activity against , when extracted with ethanol 90 GL, during 4 h, at 1:20 of D:S.