Mannitol utilisation by celery ( Apium graveolens) plants grown under different conditions in vitro

• Published in: Plant science (Limerick) Vol. 163; no. 4; pp. 907 - 916
• Main Authors: VITOVA, Lenka, STODULKOVA, Eva, BARTONICKOVA, Alena, LIPAVSKA, Helena
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.10.2002; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Carbohydrates; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Metabolism; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Osmotic stress; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; Sugar alcohol; Carbohydrates; MTD, NAD-dependent mannitol dehydrogenase; PEG, polyethylene glycol; Sugar alcohol; Osmotic stress; Culture medium; Energy metabolism; Sucrose; Growth; Use; Micropropagation; Biomass; Apium graveolens; Disaccharide; Stress; Energy source; Vegetable crop; Dicotyledones; Angiospermae; Mannitol; Spermatophyta; Chemical composition; Polyol; Umbelliferae; Biological accumulation; Photosynthesis; Osmotic pressure
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(02)00240-6

Celery plants produce sucrose and mannitol as primary photosynthetic products and translocate both carbohydrates in the phloem and metabolise them in sinks. In vitro cultivation allows the supply of plants with different exogenous carbon and energy sources, thus influencing the endogenous equilibrium of carbohydrates and allowing study of resulting changes on growth and developmental processes. We investigated the growth and metabolic responses of celery ( Apium graveolens, var. dulce, cv. Afina) to the supply of carbohydrates under in vitro conditions. No differences in biomass accumulation were observed in sucrose or mannitol supported plants at lower carbohydrate concentrations in the media (1 and 3%). Higher concentrations of mannitol, however, did not support the growth as well as media supplemented with equivalent sucrose concentrations . Differences in the degree of responses of particular organs to changes in type and concentration of media carbohydrates occurred. HPLC analysis of the soluble carbohydrate contents revealed that celery grown on mannitol accumulated very high concentrations of mannitol (65–91% of total soluble carbohydrate content). The accumulated mannitol was utilised when exogenous carbohydrates were withdrawn by transferring the plants to media without carbohydrates and the osmotic potential of the media increased.