A new approach for the study of cationic lipid–DNA complexes by energy dispersive X-ray diffraction

X-ray diffraction (XRD) studies on the cationic liposomes (CL)–DNA complexes currently used in gene delivery have provided detailed structural informations on these compact, ordered self-assemblies sheding light on the poorly understood structure–activity relationship. Among these, the experiments c...

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Bibliographic Details
Published inChemical physics letters Vol. 366; no. 3; pp. 200 - 204
Main Authors Caracciolo, G., Caminiti, R., Natali, F., Congiu Castellano, A.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 10.12.2002
Elsevier Science
Subjects
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Dna, deoxyribonucleoproteins
Fundamental and applied biological sciences. Psychology
Nucleic acids
Cationic complex
Energy dispersion
Investigation method
DNA
Liposome
Artificial membrane
X ray diffraction
Structural analysis
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Summary:X-ray diffraction (XRD) studies on the cationic liposomes (CL)–DNA complexes currently used in gene delivery have provided detailed structural informations on these compact, ordered self-assemblies sheding light on the poorly understood structure–activity relationship. Among these, the experiments carried out by using a synchrotron radiation source have showed an experimental resolution remarkably better than that achieved one by traditional in house apparatuses. Here we show a new experimental approach for the study of CL–DNA complexes, based on the employment of silicon wafers as substrates, which allows to obtain high-resolution structural informations by energy dispersive X-ray diffraction (EDXD).
ISSN:0009-2614
1873-4448
DOI:10.1016/S0009-2614(02)01454-9