Production of protein isolates from yellow mustard meals by membrane processes
Two membrane-based processes were tested for protein purification from yellow mustard meals. The starting materials were hexane-defatted meal and CH 3OH/NH 3/H 2O–hexane-extracted meal. The meals were extracted with aqueous alkali. In one process, some of the extracted protein was recovered by isoel...
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Published in | Food research international Vol. 36; no. 8; pp. 849 - 856 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
2003
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Two membrane-based processes were tested for protein purification from yellow mustard meals. The starting materials were hexane-defatted meal and CH
3OH/NH
3/H
2O–hexane-extracted meal. The meals were extracted with aqueous alkali. In one process, some of the extracted protein was recovered by isoelectric precipitation, while in the other case the alkaline extract was purified by membrane processes before isoelectric precipitation. In both processes, the protein remaining in the solution was further membrane processed, and dried to produce a soluble protein isolate. Each process recovered over 85% of Kjeldahl nitrogen present in the meal in the form of two protein isolates and a meal residue. Both protein isolates contained more than 85% protein (N×6.25), less than 0.5% phytate, and were essentially free of glucosinolates. |
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ISSN: | 0963-9969 1873-7145 |
DOI: | 10.1016/S0963-9969(03)00082-6 |