Encapsulation of Lactobacillus rhamnosus in Polyvinyl Alcohol for the production of L-(+)-Lactic Acid

• Published in: Process biochemistry (1991) Vol. 100; pp. 149 - 160
• Main Authors: Radosavljević, Miloš, Lević, Steva, Belović, Miona, Pejin, Jelena, Djukić-Vuković, Aleksandra, Mojović, Ljiljana, Nedović, Viktor
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.01.2021; Elsevier BV
• Subjects: biomass utilization; Brewing yeast; Fermentation; Fermented food; Food industry; Food processing industry; Food production; Hydrolysates; immobilization; Lactic acid; lactic acid fermentation; Lactobacilli; Lactobacillus rhamnosus; Lecithin; Low cost; Malt; Polyvinyl alcohol; Yeasts; biomass utilization; immobilization; polyvinyl alcohol; lactic acid fermentation
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2020.10.006

[Display omitted] •The polyvinyl alcohol was used in lactic acid production on renewable raw materials•PVA biocatalyst showed good fermentative stability during 4 freeze-thawing sequences•Biocatalyst reached lactic acid yield of 97% in 12 repeated batch fermentations•Biocatalyst showed high efficiency on substrate based on food industry by-products Poly(vinyl alcohol) (PVA) was investigated as a carrier for Lactobacillus rhamnosus ATCC 7469 cells for application in L-(+)-lactic acid (LA) fermentation on low-cost food industry by-products. Brewer’s spent grain (BSG) and malt rootlets (MR) hydrolysates, with the addition of brewer’s yeast, brewer’s yeast extract, or malt rootlets extract and soy lecithin were evaluated as media for LA fermentation. PVA concentrations of 5, 10 and 15% were evaluated and the optimal concentration was further used. Obtained PVA biocatalyst (BC) was applied in batch and repeated batch fermentations of BSG and MR hydrolysate. During 12 consecutive batch fermentations, the BC showed high mechanical stability and minor loss of activity while achieving high LA yield (97%) and productivity (2.1 g/L h-1). The study suggests that the immobilized L. rhamnosus cells in PVA cryogel can be successfully used in stable repeated batch fermentations on low-cost fermentation media without the loss of cell activity and LA fermentation capability.