Facile multifunctional-mode of fabricated biocompatible human serum albumin/reduced graphene oxide/Cladophora glomerata nanoparticles for bacteriostatic phototherapy, bacterial tracking and antioxidant potential

• Published in: Nanotechnology Vol. 32; no. 31; pp. 315301 - 315318
• Main Authors: Amina, Musarat, Al Musayeib, Nawal M, Alarfaj, Nawal A, El-Tohamy, Maha F, Al-Hamoud, Gadah A
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 30.07.2021
• Subjects: antioxidant activity; bacterial tracking; bacteriostatic phototherapy; bionanocomposite; photodynamic; photothermal; bacteriostatic phototherapy; photothermal; bionanocomposite; bacterial tracking; antioxidant activity; photodynamic
• ISSN: 0957-4484; 1361-6528
• DOI: 10.1088/1361-6528/abf457

To overcome multi-drug resistance in microbes, highly efficient antimicrobial substances are required that have a controllable antibacterial effect and are biocompatible. In the present study, an efficient phototherapeutic antibacterial agent, human serum albumin (HSA)/reduced graphene oxide (rGO)/ bionanocomposite was synthesized by the incorporation of rGO nanoparticles with HSA, forming protein-rGO, and decorated with a natural freshwater seaweed . The prepared HSA/rGO/ bionanocomposite was characterized by spectroscopic (UV-vis, FTIR, XRD and Raman) and microscopic (TEM and SEM) techniques. The as-synthesized bionanocomposite showed that sunlight/NIR irradiation stimulated ROS-generating dual-phototherapic effects against antibiotic-resistant bacteria. The bionanocomposite exerted strong antibacterial effects (above 96 %) against amoxicillin-resistant and , in contrast to single-model-phototherapy. The bionanocomposite not only generated abundant ROS for killing bacteria, but also expressed a fluorescence image for bacterial tracking under sunlight/NIR irradiation. Additionally, the bionanocomposite displayed pronounced antioxidant activity.