Rational design and development of a universal baby spinach-based sensing platform for the detection of biomolecules

Here, we designed and developed a Universal Baby Spinach-based Probe (UBSP) for biomolecule detection by introducing a DNA repressor containing a target recognition element. By employing different interaction modes between targets and repressors, we applied the UBSP to detect diverse classes of anal...

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Bibliographic Details
Published inAnalyst (London) Vol. 144; no. 24; pp. 7173 - 7177
Main Authors Ji, Danyang, Li, Zhaohui, Kwok, Chun Kit
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 02.12.2019
Subjects
Aptamers, Nucleotide - chemistry
Benzyl Compounds - chemistry
Biomolecules
Biosensing Techniques - methods
Blood Proteins - analysis
DNA - chemistry
DNA Probes - chemistry
Fluorescent Dyes - chemistry
G-Quadruplexes
Heavy metals
Humans
Imidazolines - chemistry
Mercury - analysis
MicroRNAs - analysis
Ribonucleic acid
RNA
RNA - chemistry
RNA Probes - chemistry
RNA Probes - genetics
Spectrometry, Fluorescence - methods
Spinach
Target recognition
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Summary:Here, we designed and developed a Universal Baby Spinach-based Probe (UBSP) for biomolecule detection by introducing a DNA repressor containing a target recognition element. By employing different interaction modes between targets and repressors, we applied the UBSP to detect diverse classes of analytes, including microRNA, proteins, and heavy metal ions. In this work, we developed a novel and universal Baby Spinach-based sensing platform for simple and robust detection of biomolecules.
Bibliography:Electronic supplementary information (ESI) available. See DOI
10.1039/c9an02061f
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2654
1364-5528
DOI:10.1039/c9an02061f