Real-time monitoring of caspase-3/8 activity by self-assembling nanofiber probes in living cells

Caspase-3/8 are key members of the cysteine-aspartyl protease family with pivotal roles in apoptosis. We have designed and synthesized self-assembling probes, Nap-GFFpYDEVD-AFC and Nap-GFFpYIETD-AFC, with fluorescence 'turn-on' properties for real-time monitoring of Caspase-3/8 activity in...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 57; no. 6; pp. 797 - 8
Main Authors Zhang, Li-Song, Xu, Hong-Lei, Xia, Ying, Bi, Jian-Peng, Zhang, Chuan-Zeng, Xi, Zhen, Li, Lu-Yuan, Zhang, Zhi-Song
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 18.01.2021
Subjects
Apoptosis
Cells (biology)
Fluorescence
Monitoring
Nanofibers
Real time
Self-assembly
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Summary:Caspase-3/8 are key members of the cysteine-aspartyl protease family with pivotal roles in apoptosis. We have designed and synthesized self-assembling probes, Nap-GFFpYDEVD-AFC and Nap-GFFpYIETD-AFC, with fluorescence 'turn-on' properties for real-time monitoring of Caspase-3/8 activity in living cells. Supramolecular self-assembling nanofiber probes Nap-GFFpYDEVD-AFC/Nap-GFFpYIETD-AFC for real-time monitoring of caspase-3/8 activity in living cells.
Bibliography:10.1039/d0cc07821b
Electronic supplementary information (ESI) available. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1359-7345
1364-548X
DOI:10.1039/d0cc07821b