Water Balance and Arginine Vasotocin in the Cocooning Frog Cyclorana platycephala (Hylidae)

• Published in: Physiological and biochemical zoology Vol. 81; no. 1; pp. 43 - 53
• Main Authors: Cartledge, Victoria A., Withers, Philip C., Bradshaw, S. Don
• Format: Journal Article
• Language: English
• Published: United States The University of Chicago Press 01.01.2008
• Subjects: Animals; Anura - physiology; Cyclorana platycephala; Estivation - physiology; Freshwater; Seasons; Time Factors; Vasotocin - metabolism; Water-Electrolyte Balance - physiology
• ISSN: 1522-2152; 1537-5293
• DOI: 10.1086/523856

It is well established that forming a cocoon, for frog species capable of doing so, markedly reduces evaporative water loss; however, the capacity of cocooned frogs to maintain hydration during extended estivation is not well understood. The combined effects of long-term estivation and water loss were examined in the cocoon-forming species Cyclorana platycephala by assessing the hydration state of the frogs throughout a 15-mo estivation period. Frogs lost mass throughout the 15-mo period to a maximum of 36% ± 6.5% of their initial standard mass. Plasma osmolality reached maximal levels by the ninth month of estivation at 487 mOsm kg−1 and then remained stable to the fifteenth month of estivation. Urine osmolality continued to increase to the fifteenth month of estivation, at which point plasma and urine concentrations were isosmotic. The use of bladder water to counter losses from circulation was indicated by the relatively slow rate of increase in plasma osmolality with mass loss and the progressive increase in urine osmolality. For estivating frogs, evidence was found for a possible threshold relationship between plasma osmolality and plasma arginine vasotocin (AVT) concentration. After estivation, plasma AVT concentrations decreased markedly after 15-mo estivators were placed in water for 2 h, suggesting that high levels of AVT may not be integral to rapid rehydration in this species.