Effect of l-Glutamine treatment on the expression of T and B cell surface molecules and secreted cytokines by cultured peripheral blood of healthy subjects

• Published in: Nutrire : revista de Sociedade Brasileira de Alimentação e Nutrição = journal of the Brazilian Society of Food and Nutrition Vol. 47; no. 2; p. 19
• Main Authors: Neves-Amorim, E. G., Santos, S. Q., Araújo-Pereira, M., Santana, Z. V. B., Bomfim, E. K. S., Chagas, N. M. B. L., Conceição, R. R., Freire, M. D. M., Torres, A. J. L., Fortuna, V., de Carvalho, G. C., Meyer, J. R., Freire, S. M., Freire, A. N. M.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 15.08.2022; Springer Nature B.V
• Subjects: Amino acids; Blood tests; Clinical Nutrition; Cytokines; Dietary supplements; Food Science; Immune system; Lymphocytes; Medicine; Medicine & Public Health; Nutrition; Glutamine dipeptide; Kinetic; Whole-blood culture; Lymphocytes; Immunomodulation; Immune system
• ISSN: 2316-7874; 1519-8928; 2316-7874
• DOI: 10.1186/s41110-022-00169-5

Background l -Glutamine ( l -GLN) is the most abundant amino acid in the human body. In hypercatabolic conditions, supplementation with l -GLN improves immunological function. Among the immune cells, the lymphocytes are the largest consumers of l -GLN in a catabolic disease state. Here, we evaluated the effect of l -GLN treatment on the expression of T and B cell surface molecules and secreted cytokines in cultured peripheral blood of healthy subjects. Methods Peripheral blood samples ( N  = 14) were cultured with or without l -GLN treatment and immunostained for T (anti-CD45, anti-CD3, anti-CD4, anti-CD8) and B (anti-CD19 and anti-CD20) lymphocytes at different time points over 24 h. In addition, secreted cytokines IL1-β, IL-6, IL-8, IL-10, IL-12p70, and TNF were measured at the same period with cytometric bead array. Results Our data showed that CD45 expression increased after 18 h in the l -GLN treatment group. On the other hand, l -GLN decreased the expression of total (CD3 + ) and helper (CD3 + CD4 + ) T cells within 24 h, reducing the expression of cytotoxic cell markers (CD3 + CD8 + ) at 12 and 18 h and of B cells (CD19 + and CD20 + ) at 18 h. Also, IL-1β and IL-10 cytokines increased at 18 and 24 h in the l -GLN treatment group supernatant of cultured peripheral blood. Conclusions The main finding of this study is that in vitro treatment with l -GLN can modulate the expression of T and B lymphocytes surface molecules and increase the secretion of IL-1β and IL-10 after 24 h.