Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker

The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at mult...

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Bibliographic Details
Published inCurrent genetics Vol. 11; no. 8; pp. 639 - 641
Main Authors Beri, R.K, Turner, G
Format Journal Article
LanguageEnglish
Published United States 01.01.1987
Subjects
acetamidase gene
Aspergillus nidulans - genetics
Aspergillus nidulellus
enzymes
genes
Genes, Dominant
Genes, Fungal
genetic transformation
Nucleic Acid Hybridization
Penicillium - genetics
Penicillium chrysogenum
Penicillium chrysogenum - genetics
stability
Transformation, Genetic
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Summary:The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00393928