DHA-enriched phospholipids from large yellow croaker roe regulate lipid metabolic disorders and gut microbiota imbalance in SD rats with a high-fat diet

• Published in: Food & function Vol. 12; no. 11; pp. 4825 - 4841
• Main Authors: Lu, Xiaodan, Zhong, Rongbin, Hu, Ling, Huang, Luyao, Chen, Lijiao, Cheng, Wenjian, Zheng, Baodong, Liang, Peng
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 08.06.2021
• Subjects: Bioinformatics; Body weight; Diet; Docosahexaenoic acid; Dosage; Fatty acids; Food; Food additives; Food intake; Functional foods & nutraceuticals; Gene sequencing; High fat diet; Histopathology; Intestinal microflora; Lipid metabolism; Lipids; Metabolic disorders; Metabolism; Microbiota; Nutritive value; Phospholipids; Polyunsaturated fatty acids; Rodents; rRNA 16S; Simvastatin; Technology assessment; Triglycerides
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d1fo00747e

Large yellow croaker roe phospholipids (LYCRPLs) have great nutritional value because they are rich in docosahexaenoic acid (DHA), which is an n-3 polyunsaturated fatty acid (n-3 PUFA). In previous research, we studied the effect of LYCRPLs on the inhibition of triglyceride accumulation at the cellular level. However, its lipid regulation effect in rats on a high-fat diet and its influence on the gut microbiota has not yet been clarified. In this study, a high-fat diet was used to induce the lipid metabolism disorder in SD rats, and simvastatin, low-dose, medium-dose and high-dose LYCRPLs were given by intragastric administration for 8 weeks. The rats' body weight, food intake, organ index, blood biochemical indicators, epididymal fat tissue and liver histopathology were compared and analyzed. High-throughput 16S rRNA gene sequencing technology and bioinformatics analysis technology were also used to analyze the diversity of gut microbiota in rats. We found that LYCRPLs can significantly regulate lipid metabolism, and improve the gut microbiota disorder induced in rats by a high-fat diet. These results can lay a foundation for the study of the regulation mechanism of LYCRPLs lipid metabolism, and also provide a theoretical basis for the development of LYCRPLs as functional food additives and excipients with hypolipidemic effects. LYCRPLs were found to significantly regulate lipid metabolism and improve the gut microbiota disorder induced in rats by a high-fat diet.