Application of direct-fed microbial bacteria and fructooligosaccharides for Salmonella control in broilers during feed withdrawal

• Published in: Poultry science Vol. 75; no. 2; pp. 186 - 190
• Main Authors: Oyarzabal, O.A. (Auburn University, AL.), Conner, D.E
• Format: Journal Article
• Language: English
• Published: England 01.02.1996
• Subjects: AGE; AGUA POTABLE; Animals; BACTERIA; Cecum - microbiology; Chickens; COMPETENCIA BIOLOGICA; COMPETITION BIOLOGIQUE; CONTEO DE CELULAS; Diet - standards; Diet - veterinary; Drinking - physiology; Eating - physiology; EAU POTABLE; EDAD; Enterococcus faecium - isolation & purification; Enterococcus faecium - physiology; ESTRES; EXPERIMENTACION; EXPERIMENTATION; FLORA MICROBIANA; FLORE MICROBIENNE; Food Deprivation - physiology; INTESTIN; INTESTINOS; Lactobacillus - isolation & purification; Lactobacillus - physiology; Lactococcus lactis - isolation & purification; Lactococcus lactis - physiology; NUMERATION CELLULAIRE; OLIGOSACARIDOS; OLIGOSACCHARIDE; Oligosaccharides - administration & dosage; Oligosaccharides - pharmacology; Pediococcus - isolation & purification; Pediococcus - physiology; POLLO DE ENGORDE; POULET DE CHAIR; Poultry Diseases - diet therapy; Poultry Diseases - physiopathology; Poultry Diseases - prevention & control; Propionibacterium - isolation & purification; Propionibacterium - physiology; SALMONELLA; Salmonella - growth & development; Salmonella - isolation & purification; Salmonella Infections, Animal - diet therapy; Salmonella Infections, Animal - physiopathology; Salmonella Infections, Animal - prevention & control; Salmonella typhimurium - isolation & purification; STRESS; Water Microbiology
• ISSN: 0032-5791; 1525-3171
• DOI: 10.3382/ps.0750186

Providing direct-fed-microbial (DFM) bacteria and fructooligosaccharides (FOS) for the control of potential escalation of Salmonella colonization during simulated feed withdrawal and confinement was assessed. Eight hundred and eighty broilers (16 pens; 55 chicks per pen) were reared to 6 wk of age. Chicks were sprayed with a solution containing 10(6) nalidixic-acid resistant Salmonella typhimurium(NR) cells per milliliter on the 2nd d after hatching. Because this first challenge did not yield a high infection rate, chickens were rechallenged per os at Day 18 by providing water containing 10(7) cells of S. typhimurium(NR) per milliliter. At 3 and 5 wk of age, 10 birds per pen were euthanatized and cecal Salmonella were quantified (log colony-forming units per gram). Feed was removed from all pens at 6 wk, and pens were randomly assigned to be either the treatment group or the control group. The treatment groups were provided a DFM (mixture of nine bacteria) and FOS 50 (10%) in the drinking water. The control groups received drinking water only. After 6 h of feed withdrawal, chickens were cooped (eight per coop) and held 10 h. Immediately after confinement, 10 chickens were used for cecal enumeration of S. typhimurium(NR). Salmonella colonization declined from 99% at 3 wk to 44% at 5 wk. After feed withdrawal, application of the treatment, and confinement, 11 and 14% of the treated and control groups, respectively, yielded S. typhimurium(NR) by direct plating from ceca (3.87 and 3.75 log10 cfu/g, respectively). No difference (P 0.05) in Salmonella colonization occurred between the treated and the control groups; however, enrichment of ceca (incubation in nutrient broth at 37 C for 24 h) yielded a higher incidence of S. typhimurium(NR) in the control groups (32% in the treated vs 51% in the control). Ceca weights were greater in the treated group (P 0.05). Simulated feed withdrawal and confinement did not escalate Salmonella colonization in the chicken ceca