Simultaneous determination of codeine, caffeine, butalbital, and aspirin by free solution capillary electrophoresis

A free solution capillary electrophoresis method was developed to separate and quantitate codeine (CD), caffeine (CF), butalbital (BB), and aspirin (AP) in a mixture. The mixture was injected hydrodynamically for 5s at the anodic end and separation was performed on a fused silica capillary (72 cm ×...

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Bibliographic Details
Published inJournal of liquid chromatography & related technologies Vol. 22; no. 8; pp. 1193 - 1204
Main Authors Haque, A, Stewart, J.T
Format Journal Article
LanguageEnglish
Published Colchester Taylor & Francis Group 1999
Taylor & Francis
Subjects
Analysis
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacology. Drug treatments
quantitative analysis
separation
Chemical analysis
Zone electrophoresis
Morphine derivatives
Capillary electrophoresis
Opiates
Acetylsalicylic acid
Mobile phase
Butalbital
Analgesic
Multicomponent analysis
Ultraviolet detector
Simultaneous measurement
Antipyretic
Dosage form
pH
Tablet
Xanthine derivatives
Salicylates
Barbiturates
Quantitative analysis
Caffeine
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Summary:A free solution capillary electrophoresis method was developed to separate and quantitate codeine (CD), caffeine (CF), butalbital (BB), and aspirin (AP) in a mixture. The mixture was injected hydrodynamically for 5s at the anodic end and separation was performed on a fused silica capillary (72 cm × 50 μm i.d; 50cm to detector) at an applied voltage of 20 kV with 0.05 M phosphate run buffer pH 8. Separation was performed at ambient temperature and the total run time for analysis was 14 min. Detection was set at 220 nm. Calibration curves were prepared for CD, CF, BB, and AP with methyl p-hydroxy benzoate as internal standard. For each analyte, the correlation coefficients was greater than 0.999 (n=4). The RSDs % of ten replicate injections for each analyte were less than 1.4 %. The method was applied to the quantitation of CF, BB, and AP in a commercial tablet. Recoveries of the drug components were in the 96-104 % range.
ISSN:1082-6076
1520-572X
DOI:10.1081/JLC-100101726