Induction of adventitious shoot regeneration in chrysanthemum as affected by the season

• Published in: In vitro cellular & developmental biology. Plant Vol. 47; no. 3; pp. 375 - 378
• Main Authors: Zalewska, Małgorzata, Lema-Rumińska, Justyna, Miler, Natalia, Gruszka, Magdalena, Dąbal, Weronika
• Format: Journal Article
• Language: English
• Published: New York Springer 01.06.2011; Springer-Verlag; Springer Nature B.V
• Subjects: Adventitious shoots; Autumn; Biomedical and Life Sciences; Cell Biology; Developmental Biology; DEVELOPMENTAL BIOLOGY/MORPHOGENESIS; Experiments; Genotype & phenotype; Immunization; Inoculation; Internodes; Leaves; Life Sciences; Mutation; Neurosciences; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant growth; Plant Sciences; Plant Tissue Culture; Plants; Seeds; Spring; Summer; Winter; x; Regeneration; Biological rhythm; (Ramat.) Kitam
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-010-9330-7

The subject of investigation was Chrysanthemum x grandiflorum (Ramat.) Kitam., 'Satinbleu'. Leaf explants and internodes were excised from the middle part of the donor sterile plant. Two methods of explant inoculation were applied: explants were placed polarly and horizontally. Modified MS medium (Murashige and Skoog 1962), supplemented with 11.4 μM indoleacetic acid and 2.7 μM 6-benzylaminopurine, were prepared to induce adventitious bud formation. Four dates of explant inoculation on the medium were tested: January 15, April 15, July 15, and October 15. Thus, regeneration occurred in winter, spring, summer, and autumn. In the present study, a more intensive regeneration in 'Satinbleu' chrysanthemum was observed in summer and autumn than in spring and winter, irrespective of the kind of explant and the inoculation method.