A dual-site controlled pH probe revealing the pH of sperm cytoplasm and screening for healthy spermatozoa

A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the...

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Published inJournal of materials chemistry. B, Materials for biology and medicine Vol. 9; no. 17; pp. 3662 - 3665
Main Authors Li, Xiaoliang, Wu, Sixian, Yu, Kangkang, Hou, Jianwen, Jiang, Chuan, Li, Kun, Chu, Zhiwen, Jiang, Xiaohui, Yu, Xiaoqi, Xu, Wenming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 05.05.2021
Subjects
Cytoplasm
Fluorescein
Intracellular
Nanoparticles
pH effects
Rhodamine
Sperm
Spermatozoa
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Summary:A dual-site controlled pH probe, which is composed of gold nanoparticles and modified with rhodamine and fluorescein derivatives, was applied to sensitively monitor intracellular pH changes in sperm. The pH probe revealed the intracellular pH of sperm under different conditions and demonstrated the lower pH in asthenozoospermia patients as compared to healthy individuals. Importantly, the pH probe can help screen for healthy sperm. A dual-site controlled pH probe was applied to sensitively monitor intracellular pH changes in spermatozoa. And it could observed the intracellular pH of sperm from asthenozoospermia patients was significantly lower than healthy individuals.
Bibliography:10.1039/d1tb00108f
Electronic supplementary information (ESI) available. See DOI
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:2050-750X
2050-7518
DOI:10.1039/d1tb00108f