Effects of extracted oil of fermented Tartary buckwheat on lipid-lowering, inflammation modulation, and gut microbial regulation in mice

• Published in: Food & function Vol. 14; no. 24; pp. 1814 - 1828
• Main Authors: Yang, Xin, Zhu, Anran, Li, Xuanchen, He, Shengling, Zhu, Yi, Anyan, Wen, Qin, Likang, Zeng, Haiying
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 11.12.2023
• Subjects: Abundance; Animals; Bacteria; Body weight; Buckwheat; Carbon dioxide; Diet, High-Fat - adverse effects; Dietary supplements; Digestive system; Disorders; Dyslipidemia; Fagopyrum - chemistry; Fagopyrum tataricum; Gastrointestinal Microbiome; Gastrointestinal tract; High density lipoprotein; High fat diet; Hyperglycemia; Inflammation; Inflammation - drug therapy; Intestine; Lipids; Liver; Low density lipoprotein; Mice; Microorganisms; Modulation; Oils & fats; Oleic acid; Palmitic acid; Relative abundance; Supercritical gas extraction; Tumor necrosis factor-α
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/d3fo04117d

This study investigated the composition of Tartary buckwheat oil fermented by Monascus purpureus and extracted under supercritical CO 2 conditions (FTBO) and evaluated its effects on lipid-lowering, inflammation modulation, and gut microbial regulation in mice that were fed a high-fat diet (MOD). Compared with the raw oil (TBO), the γ-oryzanol content reached 27.09 mg g −1 ; the monounsaturated fatty acid (MUFA) content (such as oleic acid and palmitic acid) was elevated; and the antioxidant capacities of DPPH, ABTS, and hydroxyl were improved in FTBO ( p < 0.0001). Then, supplementation with FTBO had a remarkable effect on reducing the body weight and visceral obesity as well as alleviating hyperglycemia, dyslipidemia, inflammatory reactions, and liver damage. The TC, TG, and LDL-C levels in the liver and plasma were reduced, and the HDL-C levels in the liver were increased ( p < 0.05). In particular, the high-dose group (FTBOH) exhibited the most significant effect on reducing the pro-inflammatory cytokines ET, TNF-α, IL-1β, and IL-6 in the liver, which were 18.85, 570.12, 50.47, and 26.22 pg mL −1 , respectively ( p < 0.05). Moreover, FTBO reversed intestinal disorders and increased the intestinal microbial diversity and richness. The relative abundance of beneficial bacteria, such as Bifidobacterium , Lactobacillus , Limosilactobacillus , and Lachnospiraceae_UCG-006 , were increased, and the relative abundance of the harmful bacteria Staphylococcus and Lachnoclostridium were reduced. In summary, FTBO has potential applications as a dietary supplement or dietary modifier in lowering blood lipids, modulating immune activity, and reversing intestinal disorders. This study provides reference guidance for the subsequent industrialization and development of Tartary buckwheat, the extension of the industrial chain, the development of new products, and the extraction of functional components. This study investigated the composition of Tartary buckwheat oil fermented by Monascus purpureus and extracted under supercritical CO 2 conditions (FTBO), and evaluated its effects on lipid-lowering, inflammation modulation, and gut microbial regulation in mice.