Optimization of the organic solvent-stable asymmetric hydrogen transfer system of Rhodococcus ruber DSM 44541: an activity-growth study

• Published in: Journal of molecular catalysis. B, Enzymatic Vol. 22; no. 1; pp. 1 - 6
• Main Authors: Kosjek, B., Stampfer, W., Glueck, S.M., Pogorevc, M., Ellmer, U., Wallner, S.R., Koegl, M.F., Poessl, T.M., Mayer, S.F., Ueberbacher, B., Faber, K., Kroutil, W.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2003; Elsevier Science
• Subjects: Activity-growth; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Fermentation; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Rhodococcus ruber; sec-Alcohol dehydrogenase; Activity-growth; Rhodococcus ruber; Fermentation; sec-Alcohol dehydrogenase; Microorganism growth; Enzyme; Rhodococcus; Alcohol dehydrogenase; Optimization; Organic solvent; Enzymatic activity; Bacteria; Actinomycetes; Oxidoreductases
• ISSN: 1381-1177; 1873-3158
• DOI: 10.1016/S1381-1177(02)00265-5

The organic solvent-stable redox-system of Rhodococcus ruber DSM 44541, which allows the efficient oxidation/reduction of sec-alcohols/ketones at the expense of acetone/2-propanol, respectively, as cosubstrate was optimized with respect to a maximum of alcohol dehydrogenase activity during cell growth. Comparison of the fermentation of R. ruber DSM 44541 in shake flasks cultures (1 l flask with 250 ml medium) and in a bioreactor (15 l with 10 l working volume) revealed that the desired organic solvent-stable alcohol dehydrogenase activity reached its maximum during the log phase for the bioreactor. In contrast, in shake flasks the maximum of activity was reached during the stationary phase.