Solid-state fermentation enhances inulinase and invertase production by Aspergillus brasiliensis

• Published in: Process biochemistry (1991) Vol. 108; pp. 169 - 175
• Main Authors: Guerrero-Urrutia, C., Volke-Sepulveda, T., Figueroa-Martinez, F., Favela-Torres, E.
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.09.2021; Elsevier BV
• Subjects: Aspergillus brasiliensis; Carbon dioxide; Catabolic repression; CO2 production; Extracellular enzymes; Fermentation; Inulinase; Invertase; Lag phase; Parameter estimation; Solid state; Solid state fermentation; Submerged fermentation; Sucrose; Submerged fermentation; CO2 production; Extracellular enzymes; Catabolic repression
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2021.06.014

[Display omitted] •Solid culture boosts inulinase and invertase production with no catabolic repression.•Inulinase is the preponderant enzyme under solid and submerged cultures.•Aspergillus brasiliensis growth is higher in solid than in submerged culture. Extracellular inulinase and invertase production by Aspergillus brasiliensis was evaluated in solid-state fermentation (SSF) and submerged fermentation (SmF) with a high sucrose concentration. The parameters estimated from online CO2 analysis revealed a lower Lag phase in SSF compared to SmF; the specific CO2 production rate (μCO2) decreased in both systems as the sucrose concentration increased. The highest maximum CO2 production rate (10.45 ± 0.72 mg/h mL) and total CO2 production (138.53 ± 6.48 mg/mL) were obtained in SSF with a sucrose concentration of 210 g/L. Inulinase and invertase production (1.13 ± 0.02 and 5.84 ± 0.12 U/mL, respectively) in SmF was independent of the initial sucrose concentration; meanwhile, both activities increased in SSF as the sucrose concentration increased, reaching maximum values of 5.88 ± 0.78 and 41.69 ± 3.60 U/mL, respectively, at the highest sucrose concentration (210 g/L). The zymographic analysis showed one band of inulinase and two bands of invertases involved in sucrose hydrolysis.