The laser spectroscopy of glycopolymers
The lipopolysaccharides from Ralstonia solanacearum and their structural components (O-antigen, core oligosaccharide and lipid A) and rhizobial periplasmic glucans have been investigated by laser spectroscopy. (4–8)×10 −2% water solutions of the glycopolymers were irradiated with UV laser pulses (10...
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Published in | Synthetic metals Vol. 127; no. 1; pp. 23 - 28 |
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Main Authors | , , , , , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Lausanne
Elsevier B.V
26.03.2002
Amsterdam Elsevier Science New York, NY |
Subjects | |
Online Access | Get full text |
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Summary: | The lipopolysaccharides from
Ralstonia solanacearum and their structural components (O-antigen, core oligosaccharide and lipid A) and rhizobial periplasmic glucans have been investigated by laser spectroscopy. (4–8)×10
−2% water solutions of the glycopolymers were irradiated with UV laser pulses (100
Hz, 7
ns, 337
nm). The optical reply was registered in vitro within
370–600
nm
spectral range with a photon counter equipped with a coincidence circuit. The fluorescent band profiles can help in distinguishing the different types of polysaccharides, which was impossible using optical absorption measurements due to lower sensitivity. To check the laser beam impact on biomatter the lipopolysaccharides were exposed to the laser pulses for 5
s, 5, 15, 30 and 60
min. Expositions of up to 15
min were found not to modify the biological activity, whereas 30 and 60
min exposition cause partial and total loss of activity, respectively. |
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ISSN: | 0379-6779 1879-3290 |
DOI: | 10.1016/S0379-6779(01)00591-4 |