Physico-chemical properties, kinetic parameters, and glucose inhibition of several beta-glucosidases for industrial applications

• Published in: Process biochemistry (1991) Vol. 78; pp. 82 - 90
• Main Authors: Andrades, Diandra de, Graebin, Natália G., Ayub, Marco A.Z., Fernandez-Lafuente, Roberto, Rodrigues, Rafael C.
• Format: Journal Article
• Language: English
• Published: Barking Elsevier Ltd 01.03.2019; Elsevier BV
• Subjects: Cellobiase; Cellobiose; Chemical properties; Chromium; Enzyme activity; Fruit juices; Glucose; Glucose tolerance; Glucosidase; Hydrolysis; Industrial applications; Inhibition; Kinetic parameters; Organic chemistry; Parameters; pH effects; Physicochemical properties; Stability analysis; Thermal stability; Wine; β-Glucosidase; Glucose tolerance; Enzyme activity; Kinetic parameters; β-glucosidase
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2019.01.008

[Display omitted] •β-glucosidase activity of nine commercial enzymatic preparations was compared.•Celluclast® 1.5 L showed the highest activity on pNPG.•Lallzyme Beta produced the highest yields on cellobiose hydrolysis.•Pectinex Smash XXL presented 44% of activity at 222 mmol L−1 of glucose, at pH 7.0.•Pectinex Ultra SP-L presented 54% of activity at 167 mmol L−1 of glucose, at pH 3.0. The β-glucosidase activities of nine commercial enzymatic preparations were compared by evaluating the effects of pH, thermal stability, kinetic parameters, and glucose tolerance. The pH value of the reaction medium strongly influenced the activities, stabilities, and glucose inhibition of the assayed β-glucosidases. Celluclast® 1.5 L showed the highest activity on pNPG hydrolysis (70.55 U mL−1 and 0.69 U mg−1), whereas Lallzyme Beta produced the highest yields on cellobiose hydrolysis (93.4%). Regarding the glucose tolerance and the pH, Pectinex Ultra SP-L presented the maximum glucose tolerance at pH 3.0, keeping 54% of its initial activity in the presence of 167 mmol L−1 of glucose, whereas at pH 7.0, Pectinex Smash XXL presented 44% of activity at 222 mmol L−1 of glucose. Pectinex Smash XXL showed the highest KI (19.75 mol L−1), whereas Celluclast® 1.5 L presented the highest KI/KM (19). Our results suggest that these commercial preparations are promising sources of β-glucosidases, being Pectinex Smash XXL indicated for applications at neutral pH because of its glucose tolerance and thermal stability at this pH value, whereas Celluclast® 1.5 L, Lallzyme Beta, and Pectinex Ultra SP-L are more recommended for applications at acid pH, such as in fruit juices and wine productions.